Bacteria are microscopically small, single-cell creatures having a primitive structure. The bacteria body is sealed by a relatively solid-cell membrane. Ozone interferes with the metabolism of bacterium-cells, most likely through inhibiting and blocking the operation of the enzymatic control system. A sufficient amount of ozone breaks through the cell membrane, and this leads to the destruction of the bacteria.
Viruses are small, independent particles, built of crystals and macromolecules, Unlike bacteria, they multiply only within the host cell. They transform protein of the host cell into proteins of their own. Ozone destroys viruses by diffusing through the protein coat into the nucleic acid core, resulting in damage of the viral RNA. At higher concentrations, ozone destroys the capsid, or exterior protein shell by oxidation so DNA (deoxyribonucleic acid), or RNA (ribonucleic acid) structures of the microorganism are affected.
1-mg/l = 1-PPM
| Pathogen | Dosage |
| Aspergillus Niger (Black Mount) | Destroyed by 1.5 to 2 mg/I |
| Bacillus Bacteria | Destroyed by 0.2 m/I within 30 seconds |
| Bacillus Anthracis (causes anthrax in sheep, cattle and pigs. Also a human pathogen) | Ozone susceptible |
| Candida Bacteria | Ozone susceptible |
| Clostridium Bacteria | Ozone susceptible |
| Clostridium Botulinum Spores. Its toxin paralyses the central nerve system, being a poison multiplying in food and meals. | 0.4 to 0.5 mg/l threshold value |
| Coxsackie Virus | Destroyed to zero level in less than 30 seconds by 0.1 to 0.8 mg/l |
| Diphtheria Pathogen | Destroyed by 1.5 to 2 mg/l |
| Eberth Bacillus (Typhus abdomanalis). Spreads typically by aqueous infection and causes typhoid. | Destroyed by 1.5 to 2 mg/l |
| Echo Virus 29: The virus most sensitive to ozone. | After a contact time of 1 minute at 1 mg/l of ozone, 99.999% killed. |
| Escherichia Coli Bacteria (from feces) | Destroyed by 0.2 mg/l within 30 seconds |
| Encephalomyocarditis Virus | Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l. |
| Endamoebic Cysts Bacteria | Ozone susceptible |
| Enterovirus Virus | Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l. |
| GDVII Virus | Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l. |
| Herpes Virus | Destroyed to zero level in less than 30 seconds wit 0.1 to 0.8 mg/l. |
| Influenza Virus | 0.4 to 0.5 mg/l threshold value |
| Klebs-Loffler Bacillus | Destroyed by 1.5 to 2 mg/l |
| Luminescent Basidiomycetes (species having no melanin pigment). | Destroyed in 10 minutes at 100-PPM |
| Penicillium Bacteria | Ozone susceptible |
| Poliomyelitis Virus | 99.99% kill with 0.3 to 0.4 mg/l in 3-4 minutes |
| Proteus Bacteria | Very susceptible |
| Pseudomonas Bacteria | Very susceptible |
| Rhabdovirus virus | Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l |
| Salmonella Bacteria | Very susceptible |
| Schistosoma Bacteria | Very susceptible |
| Staphylococci | Destroyed by 1.5 to 2.0 mg/l |
| Stomatitis Virus | Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l |
| Streptococcus Bacteria | Destroyed by 0.2 mg/l within 30 seconds |
| Vesicular Virus | Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l |
| Virbrio Cholera Bacteria | Very susceptible |
| Vicia Faba progeny | Ozone causes chromosome aberration and its effect is twice that observed by the action of X-rays |